愛媛大学沿岸環境科学研究センター 化学汚染・毒性解析部門:環境毒性学(岩田)研究室
Japanese
基盤研究(S)のページ

本年度成果

(1)細胞内受容体およびCYPのcDNAクローニング:無脊椎動物・魚類・両生類・鳥類・ほ乳類に属する生物種の細胞内受容体(またはそのホモログ)、およびCYP1-4ファミリーに属する分子種の全長cDNAのクローン化に成功した。また、これまでに報告されてこなかった新規の細胞内受容体・CYP分子種も新たに発見した。

(2)CYP遺伝子プロモーター/エンハンサー領域のクローニング:魚類・両生類・鳥類・ほ乳類に属する生物種のCYP遺伝子プロモーター/エンハンサー領域のクローニングに成功した。in silico系およびin vitro系での解析により、細胞内受容体特異的な反応部位を同定することができた。これらの結果により、細胞内受容体-CYPシグナル伝達系の進化および生物種特異性の一端が明らかになった。

(3)CYPによる化学物質代謝能の解明と代謝経路・産物の網羅的解析:鳥類・ほ乳類のCYP1A分子種の全長cDNAのクローンを用いて、これら遺伝子のin vitro系による蛋白質の発現に成功した。またエトキシレゾルフィンやPCBなどの化学物質(基質)とこれら蛋白質を反応させることにより、代謝産物を同定することができた。得られた代謝産物の種類・量から、進化学的近縁種であってもCYP1の代謝能は種間で大きく異なることがわかった。

(4)細胞内受容体およびCYP以外の感受性規定因子の探索:組換え近交系マウス各系統の脾細胞を2,3,7,8-四塩素化ダイオキシンで処理し、CYP1A1遺伝子発現量の用量依存性を解析したところ、マウス各系統群間でのEC50等の用量ー応答パラメーターに明確な差が認められた。これら感受性の差はマウス各系統のAHRアミノ酸配列のみでは説明できなかった。したがって、この差はAHR以外の要因が関与していると推察された。

著書

  1. Iwata, H. and Kim, E. Y. (2010):
    Risk assessment of dioxins in wild birds by the combination of contamination level and species-specific response of aryl hydrocarbon receptor, Proceeding Book "Interdisciplinary Studies on Environmental Chemistry, Vol. 3",
    Terra Scientific Publishing Company, in press.
  2. 岩田久人・金 恩英 (2009):
    第5章 生物反応を利用して化学物質汚染の危険性を検知する, 分子でよむ環境汚染(鈴木 聡編)
    東海大学出版会,pp.122-157.

原著論文

  1. Watanabe, M. X., Kunisue, T., Tao, L., Kannan, K., Subramanian, An., Tanabe, S. and Iwata, H. (2010):
    Dioxin-like and perfluorinated compounds in pigs in an Indian open waste dumping site: toxicokinetics and effects on hepatic cytochrome P450 and blood plasma hormones,
    Environmental Toxicology and Chemistry, in press.
  2. Kai, H., Ishibashi, Y., Mori, T., Ishibashi, H., Kawaguchi, T., Ohwaki, H., Arizono, K. and Takemasa, T. (2010):
    Decolorization and estrogenic activity of colored livestock wastewater by electrolysis system,
    Material Recycle and Waste Management Research, in press.
  3. Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T. B., Trang, P. T., Viet, P. H. and Tanabe, S. (2010):
    Genetic polymorphisms in glutathione S-transferase (GST) superfamily and arsenic metabolism in residents of the red river delta, vietnam,
    Toxicology and Applied Pharmacology, 242(3), 352-362.
  4. Sakai, H., Kim, E. Y., Petrov, E. A., Tanabe, S. and Iwata, H. (2009):
    Transactivation potencies of Baikal seal constitutive active/androstane receptor by persistent organic pollutants and brominated flame retardants.
    Environmental Science and Technology, 43(16), 6391-6397.
  5. Hirano, M., Ishibashi, H., Kim, J. W., Matsumura, N. and Arizono, K. (2009):
    Effects of nonylphenol on growth and 20-hydroxyecdysone levels in Mysid crustacean, Americamysis bahia.
    Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 149(3), 369-373.
  6. Kubota, A., Kim, E. Y. and Iwata, H. (2009):
    Alkoxyresorufin (methoxy-, ethoxy-, pentoxy- and benzyloxyresorufin) O-dealkylase activities by in vitro-expressed cytochrome P450 1A4 and 1A5 from common cormorant (Phalacrocorax carbo).
    Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 149(4), 544-551.
  7. Watanabe, M. X., Jones, S. P., Iwata, H., Kim, E. Y. and Kennedy, S. W. (2009):
    Effects of co-exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluorooctane sulfonate or perfluorooctanoic acid on expression of cytochrome P450 isoforms in chicken (Gallus gallus) embryo hepatocyte cultures.
    Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 149(4), 605-612.
  8. Imaeda, D., Kunisue, T., Ochi, Y., Iwata, H., Tsydenova, O., Takahashi, S., Amano, M., Petrov, E. A., Batoev, V. B. and Tanabe, S. (2009):
    Accumulation features and temporal trends of PCDDs, PCDFs and PCBs in Baikal seals (Pusa sibirica).
    Environmental Pollution, 157(3), 737-747.
  9. Lee, J. S., Kim, E. Y. and Iwata, H. (2009):
    Dioxin activation of CYP1A5 promoter/enhancer regions from two avian species, common cormorant (Phalacrocorax carbo) and chicken (Gallus gallus): Association with aryl hydrocarbon receptor 1 and 2 isoforms.
    Toxicology and Applied Pharmacology, 234(1), 1-13.
  10. Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T. B., Trang, P. T., Viet, P. H. and Tanabe, S. (2009):
    Genetic polymorphisms in AS3MT and arsenic metabolism in residents of the red river delta, Vietnam.
    Toxicology and Applied Pharmacology, 236(2), 131-141.
  11. Agusa, T., Inoue, S., Kunito, T., Minh, T. B., Ha, N. N., Tu, N. P. C., Kim Trang, P. T., Iwata, H., Viet, P. H., Tuyen, B. C. And Tanabe, S. (2009):
    Human exposure to arsenic from groundwater in the red river and mekong river deltas in Vietnam,
    International Journal of Environmental Studies, 66(1), 49-57.
  12. Agusa, T., Kunito, T., Minh, T. B., Kim Trang, P. T., Iwata, H., Viet, P. H. and Tanabe, S. (2009):
    Relationship of urinary arsenic metabolites to intake estimates in residents of the red river delta,
    Vietnam, Environmental Pollution, 157(2), 396-403.
  13. Fujihara, J., Agusa, T., Tanaka, J., Fujii, Y., Moritani, T., Hasegawa, M., Iwata, H., Tanabe, S. And Takeshita, H. (2009):
    8-hydroxy-2'-deoxyguanosine (8-OHdG) as a possible marker of arsenic poisoning: A clinical case study on the relationship between concentrations of 8-OHdG and each arsenic compound in urine of an acute promyelocytic leukemia patient being treated with arsenic trioxide,
    Forensic Toxicology, 27(1), 41-44.
  14. Kim, J. W., Ishibashi, H., Yamauchi, R., Ichikawa, N., Takao, Y., Hirano, M., Koga, M. and Arizono, K. (2009):
    Acute toxicity of pharmaceutical and personal care products on freshwater crustacean (Thamnocephalus platyurus) and fish (Oryzias latipes),
    The Journal of Toxicological Sciences, 34(2), 227-232.
  15. Kim, J. W., Jang, H. S., Kim, J. G., Ishibashi, H., Hirano, M., Nasu, K., Ichikawa, N., Takao, Y., Shinohara, R. and Arizono, K.(2009):
    Occurrence of pharmaceutical and personal care products (PPCPs) in surface water from Mankyung River,
    South Korea. Journal of Health Science, 55(2), 249-258.
  16. Yamaguchi, A., Ishibashi, H., Kohra, S., Arizono, K., Kato, K., Nakahama, T., Kanno, Y., Inouye, Y. and Tominaga, N. (2009):
    Expression analysis of estrogen-responsive genes vitellogenin 1 and 2 in liver of male medaka (Oryzias latipes) exposed to selective ligands of estrogen receptor subtypes,
    Journal of Health Science, 55(6), 930-938.
  17. Soga, Y.,Nose, M.,Arita, N.,Komor, H.,Miyazaki, T.,Maeda, T. and Furuya, K. (2009): Aneurysms of the renal arteries associated with segmental arterial mediolysis in a case of polyarteritis nodosa, Pathology Int., 59, 197-200.
  18. Yamashita, M., Iwama, N., Date, F., Chiba, R., Ebina, M., Miki, H., Yamauchi, K., Sawai, T., Nose, M., Sato, S., Takahashi, T. and Ono, M. (2009).
    Charactrization of lymphangiogenesis in various stages of idiopathic diffuse alveolar damage,
    Human pathology, 40, 542-551.
  19. Soga, Y., Komori, H., Miyazaki, T., Arita, N., Terada, M., Kamada, K., Tanaka, Y., Fujino, T., Hiasa, Y., Matsuura, B., Onji, M. and Nose, M. (2009):
    Toll-Like receptor 3 signaling induces chronic pancreatitis through the Fas/Fas ligand-mediated cytotoxicity,
    Tohoku J. Exp. Med., 217, 175-184.
  20. Tanaka, Y., Komori, H., Mori, S., Soga, Y., Tsubaki, T., Terada, M., Miyazaki, T., Fujino, T., Nakamura, S., Kanno, H., Sawasaki, T., Endo, Y. and Nose, M. (2009):
    Evaluating the role of rheumatoid factors in the development of rheumatoidarthritis in a mouse model with a newly established ELISA system,
    Tohoku J Exp Med., 220, 199-206.
  21. Kamada, K., Arita, N., Tsubaki, T., Takubo, N., Fujino, T., Soga, Y., Miyazaki, T., Yamamoto, H. and Nose, M. (2009):
    Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a shingosine analogue,
    FTY720, Pathology Int., 59, 382-389.
  22. Kamao, T., Miyazaki, T., Soga, Y., Komori, H., Terada, M., Ohashi, Y. and Nose, M. (2009):
    Genetic dissociation of dacryoadenitis and sialadenitis in a sjogren’s syndrome mouse model with common and different susceptibility gene loci,
    Invest Ophthalmol Vis Sci., 50, 3257-3265.
  23. Isobe, T., Oda, H., Takayanagi, N., Kunisue, T., Komori, H., Arita, N., Ueda, N., Nose, M., Yamada, T., Takahashi, S. and Tanabe, S. (2009):
    Hexabromocyclododecanes in human adipose tissue from Japan,
    Environ. Chem., 6, 328-333.
  24. Furukawa, H., Tohma, S., Kitazawa, H., Komori, H., Nose, M. and Ono, M. (2009):
    Role of SLAM-associated protein in the pathogenesis of autoimmune diseases and immunological disorders,
    Arch Immunol Ther Exp., 58, 37-44.
  25. 鎌田一億・有田典正・椿崇仁・宮崎龍彦・藤野貴広・能勢眞人 (2009):
    関節リウマチの滑膜増殖制御の新展開―マイクロアレーによる制御遺伝子の探索―,
    臨床と研究, 86, 686-692.
  26. 能勢眞人・小森浩章 (2009):
    モデルマウスから学ぶ血管炎のゲノム的仕組み,
    J Jpn Coll Angiol., 49, 11-16.
  27. 能勢眞人・小森浩章 (2009):
    血管炎発症のポリジーンネットワークモデル,
    リウマチ科, 42, 337-343.

学会発表

 国際学会
  1. Agusa, T., Inoue, S., Kunito, T., Minh, T. B., Tu, N. P. C., Ha, N. N., Kim Trang, P. T., Iwata, H., Tuyen, B. C., Viet, P. H. and Tanabe, S. (2010):
    Arsenic, manganese and barium contamination in groundwater and human in Vietnam,The first international conference on environmental pollution, restoration and management [SETAC A/P Joint Conference],
    Ho Chi Minh City, March, Abstruct 145.
  2. Thuruthippallil L. M., Kim, E. Y. and Iwata, H. (2009):
    Risk Assessment of dioxins in the North Pacific black-footed albatross based on CYP1A transactivation potencies of the aryl hydrocarbon receptor 1 and 2 (AhR1 and AhR2),
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 23.
  3. Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T. B., Kim Trang, P. T., Viet, P. H. and Tanabe, S. (2009):
    Association of inorganic arsenic metabolism with genetic polymorphisms of GST isoforms in North Vietnamese populations:
    Utilization of stored samples in the environmental specimen bank of Ehime University, International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 47.
  4. Yamaguchi, K., Kim, E. Y. and Iwata, H. (2009):
    Functional characterization of in vitro - expressed cytochrome P450 1A1, 1A2, and 1B1 from Baikal seal (Pusa sibirica),
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 65.
  5. Suzuki, K. and Iwata, H. (2009):
    Identification and characterization of cytochrome P450 and xenobiotics receptor genes in amphibians, Xenopus laevis and Xenopus tropicalis,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 66.
  6. Dau, P. T., Ishibashi, H., Kim, E. Y. and Iwata H. (2009):
    A preliminary study on chemical ligand screening of the constitutive androstane receptor (CAR) using a surface plasmon resonance array system,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 67.
  7. Iwata, H., Nagahama, N., Watanabe, M. and Kim, E. Y. (2009):
    Quantitative assessment of the AHR-CYP1A signaling cascase in TCDD-treated avian embryos,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 68.
  8. Iida, M., Kim, E. Y., Murakami, Y., Shima, Y. and Iwata, H. (2009):
    Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on peripheral nervous system in developing red seabream (Pugrus major) embryos,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 69.
  9. Hirano, M., Ishibashi, H., Kim, E. Y., Arizono, K. and Iwata, H. (2009):
    Molecular cloning of the ecdysone receptor from a mysid crustacean (Americamysis bahia) and development of an in vitro reporter gene assay to detect the receptor-mediated responses by chemicals,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 70.
  10. Watanabe, X., M., Kunisue, T., Tao, L., Kannan, K., Subramanian, A., Tanabe, S. and Iwata, H. (2009):
    Effects of dioxin-like and perfluorinated compounds on hepatic cytochrome P450 and plasma hormones in pigs from an indian open waste dumping site,
    International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries,
    Matsuyama, Japan, December, Abstract Book 71.
  11. Nomaru, K., Kim, E. Y., Inoue, N., Yasui, T. And Iwata, H. (2009): Intracellular localization of the AHR2 isoform from avian species, International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries, Matsuyama, Japan, December, Abstract Book 72.
  12. Ishibashi, H., Kim, E. Y., Tanabe, S. and Iwata, H. (2009):
    Molecular characterization of peroxisome proliferator-activated receptor isoforms from the Baikal seal (Pusa sibirica), International Symposium on Environmental Specimen Bank, Exploring Possibility of Setting-up ESBs in Developing Countries, Matsuyama, Japan, December, Abstract Book 73.
  13. Kim, E. Y., Lee, J. S., Inoue, N., Yasui, T. and Iwata, H. (2009):
    Functional characterization of AHR isoforms,
    AHR1, AHR2 and AHRR in avian species, 21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease,
    Tokyo, Japan, October, Abstract 160.
  14. Iwata, H., Nagahama, N., Watanabe, M. and Kim, E. Y. (2009):
    Quantitative analyses of AHR-CYP1A signaling in avian developing embryos,
    21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease,
    Tokyo, Japan, October, Abstract 161.
  15. Hirano, M., Ishibashi, H., Yamauchi, R., Kim, J.W. and Arizono, K. (2009):
    Characterization of ecdysone receptor and ultraspiracle mRNA through molting period in mysid crustacean,
    21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease,
    Tokyo, Japan, October, Abstract 171.
  16. Ishibashi, H., Yamauchi, R., Kim, J. W., Hirano, M., Mori, T., Tominaga, N. and Arizono, K. (2009):
    Transcriptional modulation of estrogen-responsive genes in male medaka (Oryzias latipes) after exposure to synthetic polycyclic musks,
    21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease,
    Tokyo, Japan, October, Abstract 171.
  17. Fujisawa, N., Tanaka, K., Yamamoto, H., Ikenaka, Y., Kim, E. Y., Lee, J. S., Iwata, H. and Ishizuka, M. (2009):
    Characterization of aryl hydrocarbon receptor in avian species,
    21st Century Advances in the Molecular Toxicology of Environmental Chemicals and Pathogenesis of Disease,
    Tokyo, Japan, October, Abstract 177.
  18. Iwata, H and Kim, E. Y. (2009):
    Disruption of aryl hydrocarbon receptor-cytochrome P450 1A signaling pathway by dioxin-like chemicals in wildlife,
    BIOLOGICAL RESPONSES TO CHEMICAL CONTAMINANTS: from molecular to community level Interdisciplinary Symposium for Young Investigators,
    Aveiro, Portugal, September, Abstract Book 27.(招待講演)
  19. Agusa, T., Iwata, H., Fujihara, J., Kunito, T., Takeshita, H., Minh, T. B., Kim, P. T., Viet, P. H. and Tanabe, S. (2009):
    Interindividual variation in arsenic metabolism in a Vietnamese population: association with single nucleotide polymorphisms in AS3MT,
    BIOLOGICAL RESPONSES TO CHEMICAL CONTAMINANTS: from molecular to community level Interdisciplinary Symposium for Young Investigators,
    Aveiro, Portugal, September, Abstract Book 30.
  20. Iwata, H and Kim, E. Y. (2009): Characterization of aryl hydrocarbon receptor-cytochrome P450 1A signaling pathway in wildlife, International Symposium on the Biomonitoring and Biomarker of Endocrine Disrupting chemicals (EDCs) in Coastal and Marine Environment, Busan, Korea, September, Abstract Book 105.(招待講演)
  21. Lee, J. S., Kim, E. Y. and Iwata, H. (2009): Functional analysis of the aryl hydrocarbon receptor repressor from chicken,
    15th International Symposium on Pollutant Responses in Marine Organisms,
    Bordeaux, France, May, Abstract Book 35.
  22. Thuruthippallil, L. M., Kim, E. Y. and Iwata, H. (2009):
    CYP1A transactivation potencies of aryl hydrocarbon receptor 1 and 2 (AHR1 and AHR2) from black-footed albatross by dioxin-like congeners,
    15th International Symposium on Pollutant Responses in Marine Organisms,
    Bordeaux, France, May, Abstract Book 284.
  23. Yamaguchi, K., Kim, E. Y. and Iwata, H. (2009):
    Catalytic function of in vitro-expressed Baikal seal CYP1A1,
    15th International Symposium on Pollutant Responses in Marine Organisms,
    Bordeaux, France, May, Abstract Book 285.
  24. Lee, J. S., Kim, E. Y. and Iwata, H. (2009):
    Functional analysis of the aryl hydrocarbon receptor repressor from chicken,
    The 66th Korean Society for Biochemistry and Molecular Biology (KSBMB) Annual Meeting 2009,
    Seoul, Korea, May, Abstract Book 234.
  25. Lee, J. S., Kim, E. Y. and Iwata, H. (2009):
    Dioxin activation of CYP1A5 promoter/enhancer regions from two avian species, common cormorant (Phalacrocorax carbo) and chicken (Gallus gallus) : association with aryl hydrocarbon receptor 1 and 2 isoforms,
    The 66th Korean Society for Biochemistry and Molecular Biology (KSBMB) Annual Meeting 2009,
    Seoul, Korea, May, Abstract Book 234.
 国内学会
  1. Thuruthippallil, L. M., Kim, E. Y. and Iwata, H. (2009):
    CYP1A transactivation potencies of the black-footed albatross AhR1 and and AhR2 by dioxins and risk assessment in the North Pacific population,
    第12回日本内分泌撹乱化学物質学会,東京,12月,要旨集42.
  2. Yamaguchi, K., Kim, E. Y., Kubota, A. and Iwata, H. (2009): Functional characterization of cytochrome P450 1A1, 1A2 and 1B1 from the Baikal seal (Pusa sibirica) heterologously expressed in yeast cells, 第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集43.
  3. Nomaru, K., Kim, E. Y., Inoue, N., Yasui, T. and Iwata, H. (2009):
    Cellular localization of the AHR isoforms (AHR1 and AHR2) from avian species,
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集121.
  4. 岩渕康平・金恩英・李鎮善・安井知子・岩田久人 (2009):
    ニワトリ新規AHR1アイソフォーム(2nd AHR1)の機能解析,
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集122.
  5. Iwata, H., Nagahama, N., Watanabe, X. M. and Kim, E. Y. (2009):
    Effects of TCDD on the AHR-CYP1A signaling cascade in avian developing embryos,
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集126.
  6. Ishibashi, H., Kim, E. Y., Tanabe, S. and Iwata, H. (2009):
    Molecular characterization of peroxisome proliferators-activated receptor β/б from the Baikal seal (Pusa sibirica),
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集127.
  7. Hirano, M., Ishibashi, H., Kim, E. Y., Arizono, K. and Iwata, H. (2009):
    Development of an in vitro reporter gene assay for screening the ecdysone receptor agonists in mysid crustacean, Americamysis bahia,
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集128.
  8. Iida, M., Kim, E. Y., Murakami, Y., Shima, Y. and Iwata, H. (2009):
    Morphological changes in peripheral nervous system by TCDD in developing red seabream (Pagrus major) embryos,
    第12回日本内分泌撹乱化学物質学会,
    東京,12月,要旨集137.
  9. Suzuki, K. and Iwata, H. (2009): Identification and expression profile of CYP1-4 genes in Xenopus laevis,
    第12回日本内分泌撹乱化学物質学会, 東京,12月,要旨集151.
  10. 竹元 望・石橋弘志・平野将司・金 俊佑・山内良子・孫 琛富・内田雅也・有薗幸司 (2009):
    環境化学物質に曝露したメダカにおけるDNAマイクロアレイを用いた遺伝子発現解析 -第1報-, 
    第12回日本内分泌撹乱化学物質学会, 
    東京, 12月, 要旨集166.
  11. 山内良子・石橋弘志・内田雅也・孫 琛富・竹元 望・竹本直道・金 俊佑・平野将司・有薗幸司 (2009):
    メダカ繁殖試験によるフッ素テロマーアルコールの影響評価, 
    第12回日本内分泌撹乱化学物質学会, 
    東京, 12月, 要旨集167.
  12. 寺岡宏樹・池田晴喜・伊藤知子・鵜飼典佳・白石順也・金 恩英・岩田久人・遠藤大二・平賀武夫 (2009):
    半網羅的ディファレンシャル・ディスプレイ法システムの構築, 
    第32回日本分子生物学会年会, 
    横浜、12月, プログラム318.
  13. 山元涼子・井口綾子・内匠正太・上田清佳・石橋弘志・冨永伸明・有薗幸司 (2009):
    線虫 C.elegans を用いた食品中機能性成分の抗酸化作用評価, 
    フォーラム2009 衛生薬学・環境トキシコロジー, 
    沖縄, 11月, 要旨251.
  14. 山口明美・石橋弘志・高良真也・有園幸司・加藤恵介・中浜隆之・菅野雄一朗・井上義雄・冨永伸明 (2009):
    ERα/ERβ選択性リガンドを用いたメダカvtg1/2の発現誘導解析, 
    フォーラム2009 衛生薬学・環境トキシコロジー, 
    沖縄, 11月, 要旨310.
  15. 能勢眞人 (2009):
    膠原病のポリジーンネットワーク(シンポジウム), 
    第37回日本臨床免疫学会総会,
    東京,11月,要旨集338.
  16. 岩田久人・金 恩英 (2009):
    環境汚染物質による野生生物のリスクを評価するための科学的方法,
    日本環境変異原学会第38回大会,
    静岡,11月,要旨集82.(招待講演)
  17. 阿草哲郎・岩田久人・藤原純子・國頭 恭・Minh, T. B.・Kim Trang, P. T. ・Viet, P. H.・竹下治男・田辺信介 (2009):
    ベトナムのヒ素汚染地域住民におけるAS3MT遺伝子多型とヒ素代謝の関係,
    第15回ヒ素シンポジウム,
    大阪,11月,要旨集52.
  18. 阿草哲郎・岩田久人・藤原純子・國頭 恭・Minh, T. B.・Kim Trang, P. T. ・Viet, P. H.・竹下治男・田辺信介 (2009):
    ベトナムにおけるAS3MT遺伝子多型とヒ素代謝能の関係,
    日本DNA多型学会第18回学術集会,
    久留米市,11月,要旨集29.
  19. 内田雅也・石橋弘志・平野将司・金 俊佑・冨永伸明・山内良子・有薗幸司 (2009):
    メダカDNAマイクロアレイを用いたエストロゲン様物質により変動する遺伝子群の網羅的解析,
    第15回日本環境毒性学会・バイオアッセイ研究会合同研究発表会,
    東京,10月,要旨集20-21.
  20. 能勢眞人 (2009):
    血管炎に関する基礎研究の進歩(シンポジウム), 第50回日本脈管学会総会,
    東京,10月,要旨集S92.
  21. 井口綾子・内匠正太・山元涼子・石橋弘志・冨永伸明・有薗幸司 (2009):
    線虫DNAマイクロアレイによるリチウムの影響評価,
    第36回日本トキシコロジー学会学術年会,
    岩手,7月,要旨集S95.
  22. 山内良子・石橋弘志・平野将司・金 俊佑・高尾雄二・有薗幸司 (2009):
    有機フッ素化合物によるヒメダカ肝臓中CYP3As発現誘導,
    第36回日本トキシコロジー学会学術年会,7月,
    岩手,要旨集S265.
  23. 阿草哲郎・岩田久人・藤原純子・國頭 恭・竹下治男・Minh, T. B.・Kim Trang, P. T.・Viet, P. H. (2009):
    ベトナム人におけるGSTスーパーファミリーの遺伝子多型と尿中ヒ素化合物プロファイルの関係,
    第20回日本微量元素学会学術集会,
    東京,7月,プログラム・抄録集163.
  24. 阿草哲郎・岩田久人・藤原純子・國頭 恭・Minh, T. B.・Kim Trang, P. T. ・Viet, P. H.・竹下治男・田辺信介 (2009):
    ベトナム人のヒ素曝露とその代謝遺伝子多型,
    第18回環境化学討論会,
    つくば市、6月,講演要旨集222.
  25. 宮崎龍彦・田中ゆき・小野栄夫・能勢眞人 (2009): オステオポンチン蛋白多型による樹状細胞抗原提示能修飾の新たなメカニズム,第98回日本病理学会総会,京都,5月,要旨集240.